https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19046 Wed 20 May 2020 07:09:22 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35009 A receptor subunits were measured by RT-PCR. Results: MBP immunostaining was increased in lobule IX of preterm males, and reduced in lobule X of preterm females when compared to their term counterparts. GAD67 staining was decreased in lobule IX and X of the preterm males, but only in lobule X of the preterm females compared to term cohorts for each sex. Internal granule cell layer width of lobule X was decreased in preterm cohorts of both sexes compared to terms. There were no differences between gestational age groups for NeuN staining, GAD67 and GAT1 protein expression as measured by western blotting, or GABA_A receptor subunits as measured by RT-PCR between preterm and term for either sex. Conclusions: The present findings suggest that components of the cerebellar GABAergic system of the ex-preterm cerebellum are disrupted. The higher expression of myelin in the preterm males may be due to a deficit in axonal pruning, whereas females have a deficit in myelination at 28 corrected days of age. Together these ongoing alterations may contribute to the neurodevelopmental and behavioural disorders observed in those born preterm.]]> Thu 30 May 2019 14:58:50 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:16534 Thu 21 Jul 2022 15:38:24 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32953 A receptor subunit composition, which may further limit neurosteroid action. The objective of this study was to determine the effects of suppression of allopregnanolone levels on the markers of development and functional outcome. Pregnant guinea pigs were treated with finasteride at a dose (25 mg/kg maternal weight) shown to suppress allopregnanolone between 60 days of gestation until delivery (term ~71 days). The cerebella from neonates, whose mothers were treated with finasteride or vehicle during pregnancy, were collected at postnatal age 8. Pups that received finasteride displayed significantly greater glial fibrillary acid protein area coverage and reduced GABA_A receptor a α₆-subunit messenger RNA within the cerebellum than pups that were exposed to vehicle. These findings indicate that loss of neurosteroid action on the foetal brain in late gestation produces prolonged astrocyte activation and reductions in GABA_A receptor a α₆-subunit expression. These changes may contribute to the long-term changes in function associated with preterm birth.]]> Thu 16 Aug 2018 13:35:57 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10763 Sat 24 Mar 2018 08:13:54 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54686 Fri 08 Mar 2024 11:53:09 AEDT ]]>